Self-assembled films containing crude extract of avocado as a source of tyrosinase for monophenol detection.
Identifieur interne : 000416 ( Main/Exploration ); précédent : 000415; suivant : 000417Self-assembled films containing crude extract of avocado as a source of tyrosinase for monophenol detection.
Auteurs : RBID : pubmed:23910293English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Catechols, Phenol, Phenols.
- chemical , chemistry : Complex Mixtures, Polypropylenes, Tin Compounds.
- chemical , metabolism : Monophenol Monooxygenase.
- enzymology : Persea.
- methods : Biosensing Techniques.
- Potentiometry, Solutions, Spectrophotometry, Ultraviolet.
Abstract
This paper reports on the use of the crude extract of avocado (CEA) fruit (Persea americana) as a source of tyrosinase enzyme. CEA was immobilized via layer by layer (LbL) technique onto indium tin oxide (ITO) substrates and applied in the detection of monophenol using a potentiometric biosensor. Poly(propylene imine) dendrimer of generation 3 (PPI-G3) was used as a counter ion in the layer by layer process due to its highly porous structure and functional groups suitable for enzyme linkage. After the immobilization of the crude CEA as multilayered films, standard samples of monophenol were detected in the 0.25-4.00 mM linear range with approximately 28 mV mM(-1) of sensitivity. This sensitivity is 14 times higher than the values found in the literature for a similar system. The results show that it is possible to obtain efficient and low-cost biosensors for monophenol detection using potentiometric transducers and alternative sources of enzymes without purification.
DOI: 10.1016/j.msec.2013.05.028
PubMed: 23910293
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Self-assembled films containing crude extract of avocado as a source of tyrosinase for monophenol detection.</title><author><name sortKey="Vieira, Nirton C S" uniqKey="Vieira N">Nirton C S Vieira</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Física de São Carlos/Universidade de São Paulo, CP 369, 13560-970 São Carlos, SP, Brazil. nirtoncristi@gmail.com</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Instituto de Física de São Carlos/Universidade de São Paulo, CP 369, 13560-970 São Carlos, SP</wicri:regionArea></affiliation></author><author><name sortKey="Ferreira, Reginaldo A" uniqKey="Ferreira R">Reginaldo A Ferreira</name></author><author><name sortKey="Da Cruz Rodrigues, Valquiria" uniqKey="Da Cruz Rodrigues V">Valquiria da Cruz Rodrigues</name></author><author><name sortKey="Guimar Es, Francisco E G" uniqKey="Guimar Es F">Francisco E G Guimarães</name></author><author><name sortKey="De Queiroz, Alvaro A A" uniqKey="De Queiroz A">Alvaro A A de Queiroz</name></author></titleStmt><publicationStmt><date when="2013">2013</date><idno type="doi">10.1016/j.msec.2013.05.028</idno><idno type="RBID">pubmed:23910293</idno><idno type="pmid">23910293</idno><idno type="wicri:Area/Main/Corpus">000480</idno><idno type="wicri:Area/Main/Curation">000480</idno><idno type="wicri:Area/Main/Exploration">000416</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensing Techniques (methods)</term><term>Catechols (analysis)</term><term>Complex Mixtures (chemistry)</term><term>Monophenol Monooxygenase (metabolism)</term><term>Persea (enzymology)</term><term>Phenol (analysis)</term><term>Phenols (analysis)</term><term>Polypropylenes (chemistry)</term><term>Potentiometry</term><term>Solutions</term><term>Spectrophotometry, Ultraviolet</term><term>Tin Compounds (chemistry)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Catechols</term><term>Phenol</term><term>Phenols</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Complex Mixtures</term><term>Polypropylenes</term><term>Tin Compounds</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Monophenol Monooxygenase</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Persea</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Potentiometry</term><term>Solutions</term><term>Spectrophotometry, Ultraviolet</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This paper reports on the use of the crude extract of avocado (CEA) fruit (Persea americana) as a source of tyrosinase enzyme. CEA was immobilized via layer by layer (LbL) technique onto indium tin oxide (ITO) substrates and applied in the detection of monophenol using a potentiometric biosensor. Poly(propylene imine) dendrimer of generation 3 (PPI-G3) was used as a counter ion in the layer by layer process due to its highly porous structure and functional groups suitable for enzyme linkage. After the immobilization of the crude CEA as multilayered films, standard samples of monophenol were detected in the 0.25-4.00 mM linear range with approximately 28 mV mM(-1) of sensitivity. This sensitivity is 14 times higher than the values found in the literature for a similar system. The results show that it is possible to obtain efficient and low-cost biosensors for monophenol detection using potentiometric transducers and alternative sources of enzymes without purification.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">23910293</PMID><DateCreated><Year>2013</Year><Month>08</Month><Day>05</Day></DateCreated><DateCompleted><Year>2014</Year><Month>02</Month><Day>25</Day></DateCompleted><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-0191</ISSN><JournalIssue CitedMedium="Internet"><Volume>33</Volume><Issue>7</Issue><PubDate><Year>2013</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Materials science & engineering. C, Materials for biological applications</Title><ISOAbbreviation>Mater Sci Eng C Mater Biol Appl</ISOAbbreviation></Journal><ArticleTitle>Self-assembled films containing crude extract of avocado as a source of tyrosinase for monophenol detection.</ArticleTitle><Pagination><MedlinePgn>3899-902</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.msec.2013.05.028</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0928-4931(13)00316-0</ELocationID><Abstract><AbstractText>This paper reports on the use of the crude extract of avocado (CEA) fruit (Persea americana) as a source of tyrosinase enzyme. CEA was immobilized via layer by layer (LbL) technique onto indium tin oxide (ITO) substrates and applied in the detection of monophenol using a potentiometric biosensor. Poly(propylene imine) dendrimer of generation 3 (PPI-G3) was used as a counter ion in the layer by layer process due to its highly porous structure and functional groups suitable for enzyme linkage. After the immobilization of the crude CEA as multilayered films, standard samples of monophenol were detected in the 0.25-4.00 mM linear range with approximately 28 mV mM(-1) of sensitivity. This sensitivity is 14 times higher than the values found in the literature for a similar system. The results show that it is possible to obtain efficient and low-cost biosensors for monophenol detection using potentiometric transducers and alternative sources of enzymes without purification.</AbstractText><CopyrightInformation>Copyright © 2013 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Vieira</LastName><ForeName>Nirton C S</ForeName><Initials>NC</Initials><Affiliation>Instituto de Física de São Carlos/Universidade de São Paulo, CP 369, 13560-970 São Carlos, SP, Brazil. nirtoncristi@gmail.com</Affiliation></Author><Author ValidYN="Y"><LastName>Ferreira</LastName><ForeName>Reginaldo A</ForeName><Initials>RA</Initials></Author><Author ValidYN="Y"><LastName>da Cruz Rodrigues</LastName><ForeName>Valquiria</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Guimarães</LastName><ForeName>Francisco E G</ForeName><Initials>FE</Initials></Author><Author ValidYN="Y"><LastName>de Queiroz</LastName><ForeName>Alvaro A A</ForeName><Initials>AA</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>05</Month><Day>18</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Mater Sci Eng C Mater Biol Appl</MedlineTA><NlmUniqueID>101484109</NlmUniqueID><ISSNLinking>0928-4931</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Catechols</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Complex Mixtures</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Phenols</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Polypropylenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Solutions</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Tin Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>poly(propyleneimine)</NameOfSubstance></Chemical><Chemical><RegistryNumber>339NCG44TV</RegistryNumber><NameOfSubstance>Phenol</NameOfSubstance></Chemical><Chemical><RegistryNumber>71243-84-0</RegistryNumber><NameOfSubstance>indium tin oxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.14.18.1</RegistryNumber><NameOfSubstance>Monophenol Monooxygenase</NameOfSubstance></Chemical><Chemical><RegistryNumber>LF3AJ089DQ</RegistryNumber><NameOfSubstance>catechol</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Biosensing Techniques</DescriptorName><QualifierName MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Catechols</DescriptorName><QualifierName MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Complex Mixtures</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Monophenol Monooxygenase</DescriptorName><QualifierName MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Persea</DescriptorName><QualifierName MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Phenol</DescriptorName><QualifierName MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Phenols</DescriptorName><QualifierName MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Polypropylenes</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Potentiometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Solutions</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Spectrophotometry, Ultraviolet</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Tin Compounds</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Biosensor</Keyword><Keyword MajorTopicYN="N">Crude extract</Keyword><Keyword MajorTopicYN="N">Layer by layer</Keyword><Keyword MajorTopicYN="N">Monophenol</Keyword><Keyword MajorTopicYN="N">Tyrosinase</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>11</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2013</Year><Month>4</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>5</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2013</Year><Month>5</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>8</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>8</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>2</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pii">S0928-4931(13)00316-0</ArticleId><ArticleId IdType="doi">10.1016/j.msec.2013.05.028</ArticleId><ArticleId IdType="pubmed">23910293</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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